* What kind of patient samples are used for the purpose of identifying possible pathogens?
* What does PCR do, how does it work, and why is it useful?
* How do you separate the desired DNA from all others?
* How does an automatic DNA sequencer work?
* Why is it possible to use a DNA sequence to identify bacteria?
1. Fluid from Lymph Node, Stool Sample, Urine Sample, Blood Sample, Sputum Sample, and Stool Sample from a Child.
2. PCR which is polymerase chain reaction is a scientific technique in molecular biologyto amplify a single or a few copies of a piece of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence. It's an inexpensive technique that can make segments of DNA. PCR can target specifics parts of DNA. This is useful for analyzing DNA especially for evidence taken at a crime scene.
3. A desired sequence is isolated by using restriction enzymes. They are enzymes that cut DNA at specific nucleotide sequences. PCR envolves a mixture of lots of stuff, but essentially, you are using a cell called a competent cell to preform a transformation. The cut DNA is introduced into the cell which acts like a machine to copy that DNA over and over again. PCR means Polymerase Chain reaction. Polymerase is the enzyme reponsible for DNA replication.
4. Automated deoxyribonucleic acid (DNA) sequencing reduces the volume of low-level radioactive waste generated on campus, while providing a suitable alternative to manual DNA sequencing. Traditional methods of manual DNA sequencing utilize radioactive isotopes to label the DNA. Automated DNA sequencing utilizes fluorescent tracers instead of radioisotopes to label the DNA, thereby eliminating or significantly reducing the use of radioactive materials in some research laboratories.
5. It is possible because each strain of bacteria have their own specialized DNA sequence.
ID bacteria
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